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<p> Is there some specipic position?</p>
<p> </p>
<p>A : It is not only one answer but it can get some idea.</p>
<p>http://www6.appliedbiosystems.com/support/tutorials/pdf/quant_pcr.pdf</p>
<p>Reference : Creating Standard Curves with Genomic DNA or Plasmid DNA Templates for Use in Quantitative PCR</p>
<p> </p>
