Difference between revisions of "About sequencing"
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| − | <li> | + | <li>[Library preparation]</li> |
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| − | <p> | + | <p>[Template preparation]</p> |
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| − | <p> | + | <p>[Sequencing and imaging]</p> |
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| + | <p>Library preparation</p> | ||
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| + | <p><span style="color:#000000">A genomic library is a collection of the total genomic DNA</span><span style="color:#000000"> from a single organism</span><span style="color:#000000">. The DNA is stored in a population of identical vectors,</span><span style="color:#000000"> each containing a different insert </span><span style="color:#000000">of DNA. In order to construct a genomic library, the organism's DNA is extracted </span><span style="color:#000000">from cells </span><span style="color:#000000">and then digested with a restriction enzyme </span><span style="color:#000000">to cut the DNA into fragments of a specific size.</span></p> | ||
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Revision as of 17:56, 4 October 2017
Sequencing is consist of three steps
- [Library preparation]
-
[Template preparation]
-
[Sequencing and imaging]
Library preparation
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size.
Template preparation
Sequencing and imaging
