View source for Telomeromics

<p>A wide variety of studies show the association of short telomeres with age related pathologies and cancer, as well as with lifespan and mortality.</p>

<p>These facts highlight the importance of measuring&nbsp;telomere&nbsp;length in human populations and by using reliable methods to uncover the association between&nbsp;telomere&nbsp;length and human disease.</p>

<p>The length of telomeres, and in particular the abundance of short telomeres, has been proposed as a biomarker of aging and of general health status.</p>

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<p>Telomere length studies were started around 1980s and were initially based on Southern blot quantification of a smear of telomere restriction fragments, the so-called terminal restriction fragment (TRF) analysis</p>

<p>TRF analysis provides a rough estimate of the average number of telomeric repeats per sample, as terminal restriction fragments include not only telomeric repeats but also variable amounts of subtelomeric sequences</p>

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<p>the development of telomere measurement methods based on telomeric Fluorescence In Situ Hybridization (FISH) solved the problem of accuracy and represented a breakthrough for telomere length quantification</p>

<p>The first telomere FISH based method developed was the so-called telomere quantitative FISH method or Q-FISH on metaphases which allows telomere length quantification of individual chromosome ends at the single-cell level, providing a quantification of both very short/undetectable telomeres and of the mean telomere length per cell</p>

<p>&nbsp;However, the conventional Q-FISH method is very time consuming and labor intensive, and therefore, not suitable for high-throughput (HT) assays</p>

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<p>Reference</p>

<p><a href="http://www.aging-us.com/article/SKg3CwiAcA7AgNETc/text">http://www.aging-us.com/article/SKg3CwiAcA7AgNETc/text</a></p>

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<p>[[20131571 조우빈]]</p>