View source for Sanger sequencing


<span style="font-size:22px;">'''1<sup>st</sup> sequencing – Sanger sequencing'''</span>

*'''Sanger sequencing by capillary electrophoresis is the gold-standard DNA sequencing technique.''' 
*'''Based on the selective incorporation of chain-terminating ddNTP by DNA polymerase during in vitro DNA replication.''' 
*'''requires a single-stranded DNA template, a DNA&nbsp;primer, a&nbsp;DNA polymerase, normal deoxynucleosidetriphosphates (dNTPs), and modified di-deoxynucleosidetriphosphates (ddNTPs)''' 
*'''resulted DNA fragments are heat denatured and separated by size using gel electrophoresis''' 
*'''DNA polymerases copy single-stranded DNA templates by adding nucleotides to a growing chain. Chain elongation occurs at the 3' end of a primer, an oligonucleotide that anneals to the template. The deoxynucleotides added to the extension product are selected by base-pair matching to the template.''' 
*'''Thermal cycling -> purification -> capillary electrophoresis''' 

[http://media.invitrogen.com.edgesuite.net/ab/applications-technologies/pharma-biotherapeutics/DNA_sequencing.swf '''http://media.invitrogen.com.edgesuite.net/ab/applications-te'''chnologies/pharma-biotherapeutics/DNA_sequencing.swf]

[https://en.wikipedia.org/wiki/Sanger_sequencing https://en.wikipedia.org/wiki/Sanger_sequencing]