View source for KSH 0604 Proteomics

<p>Biological thinking =&gt; Problem - hypothesis - strategies - experimental designs - how you excuted team plans - scientific results</p>

<p>Proteomics</p>

<p>1. What is proteome?</p>

<p>The proteome is the entire set of proteins expressed by a genome, cell, tissue, or organism at a certain time</p>

<p>It is the set of expressed proteins in a given type of cell or organism, at a given time, under defined conditions</p>

<p>Proteins + Genome = Proteome</p>

<p>(Cellular proteome in a particular cell type + Viral proteome in a virus = Complete proteome)</p>

<p>Marc Wilkins used the term proteome to describe the entire complement of proteins expressed by a genome, cell, tissue or organism.</p>

<p>Knowledge of the proteome requires</p>

<p>1. the sequences of proteins</p>

<p>2. the structure of the proteins</p>

<p>3. the funcional interaction between the proteins</p>

<p>Expereiments to study proteome</p>

<p>1. 2D gel electrophoresis - to separate proteins by charge and size</p>

<p>2. Mass spectrometry<br />
(Peptide mass fingerprinting ? / Tandem mass spectrometry ?)</p>

<p>(<a href="https://en.wikipedia.org/wiki/Proteome">https://en.wikipedia.org/wiki/Proteome</a>)</p>

<p><br />
2. Relationship between genomics, transcriptomics, and proteomics</p>