View source for Helicase dependent amplification

<h2>Abstract</h2>

<p>Polymerase chain reaction is the most widely used method for&nbsp;<em>in vitro</em>&nbsp;DNA amplification. However, it requires thermocycling to separate two DNA strands.&nbsp;<em>In vivo</em>, DNA is replicated by DNA polymerases with various accessory proteins, including a DNA helicase that acts to separate duplex DNA. We have devised a new&nbsp;<em>in vitro</em>&nbsp;isothermal DNA amplification method by mimicking this&nbsp;<em>in vivo</em>&nbsp;mechanism. Helicase-dependent amplification (HDA) utilizes a DNA helicase to generate single-stranded templates for primer hybridization and subsequent primer extension by a DNA polymerase. HDA does not require thermocycling. In addition, it offers several advantages over other isothermal DNA amplification methods by having a simple reaction scheme and being a true isothermal reaction that can be performed at one temperature for the entire process. These properties offer a great potential for the development of simple portable DNA diagnostic devices to be used in the field and at the point-of-care.</p>

<p><strong>Keywords:&nbsp;</strong>DNA amplification, isothermal, helicase, DNA polymerase, UvrD</p>

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<p><strong>Reference</strong></p>

<p>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1249482/</p>

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