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<p>X35</p>
<p> [[File:14.png|400px]]</p>
<p> </p>
<p> </p> <p> <Colony PCR></p>
<p>-> Check whether insert base pairs is inserted in T vector well</p>
<p>XL-Taq polymerase: 0.5</p>
<p> ---------------------------------</p>
<p> [[File:15.jpg|400px]]</p>
<p>Can check on 3,5 well(T vector 200bp+ 346bp = 500~600bp)</p>
<p> </p>
<p> (3)</p>
<p> </p>
