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<p>New methods include protein microarrays, <u><strong>immunoaffinity chromatography followed by mass spectrometry(MALDI-TOF mass spectrometry), </strong> </u>and combinations of experimental methods such as phage display and computational methods.</p>
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<p><strong><u>Therefore, Focus more on protemoics, Metabolome!</u></strong></p>
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<p><strong><span style="font-size:14px">Adipose tissue</span></strong></p>
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<p><strong>-> Adipokine </strong></p>
<p> -> <span style="font-size:12px"><span style="color:black; font-family:맑은 고딕">Adipose tissue secreted multiple mediator</span></span></p>
<p><span style="font-size:12px"><span style="color:black; font-family:맑은 고딕"> </span></span>-> Passed through either endocrine or paracrine</p>
<p> Ex: Hormone: leptin, adiponectin</p>
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<p><strong>-> Adiponectin</strong></p>
<p><strong> -> </strong>Adipocyte-secreted adipokine</p>
<p> -> Increase lipid oxidation& anti-inflammatory, insulin-sensitizing, angiogenic action</p>
<p> <strong>=> Anti obesity & Antidiabetic, Decrease insulin resistance </strong></p>
<p> [[File:1.png|400px]]</p>
<p> </p>
<p> </p>
<p> [[File:2.gif|400px]]</p>
<p>-> Illustration of the major physiological and metabolic</p>
<p>processes with which adipose tissue is involved through the secretion</p>
<p>of various adipokines from adipocytes. The interactions may be</p>
<p>autocrine, paracrine, or endocrine.</p>
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<p><span style="font-size:16px"><strong><Searching Scientific Reports></strong></span></p>
<p> [[File:3.png|400px]]</p>
<p> [[File:4.png|400px]]</p>
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<p><strong><span style="font-size:16px"><What is Col6?></span></strong></p>
<p>- COL6 = Collagen type 6</p>
<p>- Abundant constituent of white adipose tissue (WAT)</p>
<p>- COL6 levels positively correlate with hyperglycaemia and insulin resistance</p>
<p>- Composed of three distinct a chains, a1, a2 and a3.(COL6 trimeric building block) and are subsequently secreted into the ECM</p>
<p> </p>
<p><strong><span style="font-size:16px"><What is a3 Chain?></span></strong></p>
<p>-> longest of the three chains</p>
<p>- contains an unusually long N terminus and a globular C5 domain at the C-terminus</p>
<p> </p>
<p>-> C-terminal portion of the a3 subunit is cleaved off during the post-translational processing of COL6 fibrils(COL6a3, Endotrophin)</p>
<p> </p>
<p><strong><span style="font-size:16px"><What is Endotrophin?></span></strong></p>
<p>- Adipokine with potent tumour-promoting effects</p>
<p>- Plays a pivotal role in shaping a metabolically unfavorable microenvironment in adipose tissue during consumption of a high-fat diet (HFD)</p>
<p>- Powerful co-stimulator of pathologically relevant pathways within the ‘unhealthy’ adipose tissue milieu, triggering fibrosis and inflammation and ultimately leading to enhanced insulin resistance& metabolic dysfunction.</p>
<p>- Exerts a major influence in adipose tissue</p>
<p>- Endotrophin within the tumor microenvironment serves as a major mediator of COL6-stimulated mammary tumor growth and subsequent chemo resistance</p>
<p>- Stimulates fibrosis, activates endothelial cell migration and promotes macrophage infiltration into growing solid tumors.</p>
<p>=> elevated mammary tumor expansion and more pronounced metastatic growth</p>
<p> [[File:5.png|400px]]</p>
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<p><u><strong><span style="font-size:18px">Problem!</span></strong></u></p>
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<p><strong>-> Don’t know the mechanism of how ETP works.</strong></p>
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<p><u><strong><span style="font-size:16px">What I’m going to do!</span></strong></u></p>
<p> </p>
<div><strong>-> Find the Receptor according to the New method of Protemoics.</strong></div>
<div><strong>-> Find the interaction, relationship and mechanisms how they act.(Study of Omics)</strong></div>
<div><u><strong> -> Omics could be applied to genomics perspective!</strong></u></div>
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