Changes
From Biolecture.org
no edit summary
<p><span style="color:#000000">In genomics, sequencing is to determine the arrangement </span><span style="color:#000000">of DNA. </span>Sequencing is consist of methods are outlined with three basic steps as outlined shown below.</p>
<p> </p>
<p><span style="color:#000000">A genomic library is a collection of the total genomic DNA</span><span style="color:#000000"> from a single organism</span><span style="color:#000000">. The DNA is stored in a population of identical vectors,</span><span style="color:#000000"> each containing a different insert </span><span style="color:#000000">of DNA. In order to construct a genomic library, the organism's DNA is extracted </span><span style="color:#000000">from cells </span><span style="color:#000000">and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. (above definition is by https://en.wikipedia.org/wiki/Genomic_library)</span></p>
<p><font color="#000000">Fragments made by restriction enzyme undergo additional process, such as ligation of adaptor and primer, which let them be able to function in next phase.</font></p>
<p> </p>
<h2>emPCR</h2>
<p><img alt="" src="/ckfinder/userfiles/images/emPCR%2C%20Solid-phase%20amplification%20(2).png" style="height:183px; width:731px" /></p>
<p> </p>
<p> </p>
<h2>Solid-phase</h2>
<p><img alt="" src="/ckfinder/userfiles/images/emPCR%2C%20Solid-phase%20amplification%20(3).png" style="height:285px; width:478px" /></p>
<p> </p>
