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<p><span style="font-family:comic sans ms,cursive"><span style="font-size:14px">DNA sequencing is the process of determining the nucleotide order of a given fragment.</span></span></p>
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<p><span style="font-family:comic sans ms,cursive"><span style="font-size:14px">1. Sanger sequencing</span></span></p>
<p><span style="font-size:14px"><span style="font-family:comic sans ms,cursive">Sanger sequencing is a method of DNA sequencing based on the selective incorporation of chain-terminating dideoxynucleotides(ddNTPs) by DNA polymerase during in vitro DNA replication. This method was developed by Fredric Sanger and colleagues in 1977. </span></span></p>
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<p><span style="font-size:14px"><span style="font-family:comic sans ms,cursive">2. Next-generation sequencing (NGS)</span></span></p>