Difference between revisions of "Transcriptomics Class"

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<p><span style="font-size: large">Transcriptomics class for UNIST 2015</span></p>
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<p><span style="font-size:22px">[&nbsp;Transcriptomics class ]&nbsp;</span></p>
<p><span style="font-size: medium">1. What is transcriptomics?</span></p>
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<p><span style="font-size: medium">2. Relationship between genomics and transcriptomics</span></p>
 
<p><span style="font-size: medium">3. What are mRNAs?</span></p>
 
<p><span style="font-size: medium">4. How to measure RNA expression?</span></p>
 
<p><span style="font-size: medium">5. Relationship between Transcriptome and Proteome.</span></p>
 
<p><span style="font-size: medium">6. What is a UTR?</span></p>
 
<p><span style="font-size: medium">7. What is ncRNA ?</span></p>
 
<p><span style="font-size: medium">8. What is poly A ?</span></p>
 
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
<p><span style="font-size: medium">&nbsp;</span></p>
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<p><span style="font-size: medium">What to do:</span></p>
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<p><span style="font-size:16px"># Transcriptome?&nbsp;</span></p>
<p><span style="font-size: medium">1. Apply [[Biology learning procedure]]</span></p>
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<p><span style="font-size: medium">&nbsp;</span></p>
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<p>&nbsp; = set of all mRNA molecules that are produced by the gemone, under specific circumstances in one cell or a population of cells&nbsp;</p>
 +
 
 +
<p>&nbsp; = can vary depending on environmental conditions</p>
 +
 
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<p>&nbsp; &nbsp; &nbsp;&rArr; reflects genes which are being actively expressed with the exception of mRNA degradation&nbsp;</p>
 +
 
 +
<p><span style="font-size:16px">#&nbsp;<span style="line-height:25.6px">Transcriptomics?</span></span></p>
 +
 
 +
<p>&nbsp; =&nbsp;the study of transcriptome&nbsp;</p>
 +
 
 +
<p>&nbsp; =&nbsp;referred to as expression profiling</p>
 +
 
 +
<p>&nbsp; =&nbsp;examines the expression level of mRNAs in a given cell population, often using high-throughput techniques based on&nbsp;DNA&nbsp;microarray&nbsp;technology.&nbsp;</p>
 +
 
 +
<p><span style="font-size:medium">2. Relationship between genomics and transcriptomics</span></p>
 +
 
 +
<p><span style="font-size:medium">3. What are mRNAs?</span></p>
 +
 
 +
<p><span style="font-size:medium">4. How to measure RNA expression?</span></p>
 +
 
 +
<p><span style="font-size:medium">5. Relationship between Transcriptome and Proteome.</span></p>
 +
 
 +
<p><span style="font-size:medium">6. What is a UTR?</span></p>
 +
 
 +
<p><span style="font-size:medium">7. What is ncRNA ?</span></p>
 +
 
 +
<p><span style="font-size:medium">8. What is poly A ?</span></p>
 +
 
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
<p><span style="font-size: large">Reference</span></p>
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 +
<p><span style="font-size:medium">&nbsp;</span></p>
 +
 
 +
<p><span style="font-size:medium">What to do:</span></p>
 +
 
 +
<p><span style="font-size:medium">1. Apply [[Biology learning procedure]]</span></p>
 +
 
 +
<p><span style="font-size:medium">&nbsp;</span></p>
 +
 
 +
<p>&nbsp;</p>
 +
 
 +
<p><span style="font-size:large">Reference</span></p>
 +
 
 
<p><a href="http://www.biomedcentral.com/1471-2164/13/473">http://www.biomedcentral.com/1471-2164/13/473</a></p>
 
<p><a href="http://www.biomedcentral.com/1471-2164/13/473">http://www.biomedcentral.com/1471-2164/13/473</a></p>
 +
 
<p>https://www.youtube.com/watch?v=UYJfM2-Y7y8 &nbsp;(Youtube by Illumina Gary Schroth)</p>
 
<p>https://www.youtube.com/watch?v=UYJfM2-Y7y8 &nbsp;(Youtube by Illumina Gary Schroth)</p>
 +
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
 +
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
 +
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
 +
 
<h1>Whole transcriptome analyses of six thoroughbred horses before and after exercise using RNA-Seq</h1>
 
<h1>Whole transcriptome analyses of six thoroughbred horses before and after exercise using RNA-Seq</h1>
<div class="multipleins" sizset="false" sizcache04151915416476371="85 137 107">
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<p class="authors" sizset="false" sizcache04151915416476371="85 137 107"><strong>Kyung-Do Park</strong><sup sizset="false" sizcache04151915416476371="85 137 107"><a jquery18205432795217567932="31" href="http://www.biomedcentral.com/1471-2164/13/473/#ins1">1</a></sup><sup>&dagger;</sup>, <strong>Jongsun Park</strong><sup sizset="false" sizcache04151915416476371="85 137 108"><a jquery18205432795217567932="33" href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup><sup>&dagger;</sup>, <strong>Junsu Ko</strong><sup sizset="false" sizcache04151915416476371="85 137 109"><a jquery18205432795217567932="34" href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup><sup>&dagger;</sup>, <strong>Byung Chul Kim</strong><sup sizset="false" sizcache04151915416476371="85 137 110"><a jquery18205432795217567932="35" href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup><sup sizset="false" sizcache04151915416476371="85 137 111"><a jquery18205432795217567932="36" href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup>, <strong>Heui-Soo Kim</strong><sup sizset="false" sizcache04151915416476371="85 137 112"><a jquery18205432795217567932="37" href="http://www.biomedcentral.com/1471-2164/13/473/#ins4">4</a></sup>, <strong>Kung Ahn</strong><sup sizset="false" sizcache04151915416476371="85 137 113"><a jquery18205432795217567932="38" href="http://www.biomedcentral.com/1471-2164/13/473/#ins4">4</a></sup>, <strong>Kyoung-Tag Do</strong><sup sizset="false" sizcache04151915416476371="85 137 114"><a jquery18205432795217567932="39" href="http://www.biomedcentral.com/1471-2164/13/473/#ins5">5</a></sup>, <strong>Hansol Choi</strong><sup sizset="false" sizcache04151915416476371="85 137 115"><a jquery18205432795217567932="40" href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup>, <strong>Hak-Min Kim</strong><sup sizset="false" sizcache04151915416476371="85 137 116"><a jquery18205432795217567932="41" href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup>, <strong>Sanghoon Song</strong><sup sizset="false" sizcache04151915416476371="85 137 117"><a jquery18205432795217567932="42" href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup>, <strong>Sunghoon Lee</strong><sup sizset="false" sizcache04151915416476371="85 137 118"><a jquery18205432795217567932="43" href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup>, <strong>Sungwoong Jho</strong><sup sizset="false" sizcache04151915416476371="85 137 119"><a jquery18205432795217567932="44" href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup>, <strong>Hong-Sik Kong</strong><sup sizset="false" sizcache04151915416476371="85 137 120"><a jquery18205432795217567932="45" href="http://www.biomedcentral.com/1471-2164/13/473/#ins1">1</a></sup>, <strong>Young Mok Yang</strong><sup sizset="false" sizcache04151915416476371="85 137 121"><a jquery18205432795217567932="46" href="http://www.biomedcentral.com/1471-2164/13/473/#ins6">6</a></sup>, <strong>Byung-Hak Jhun</strong><sup sizset="false" sizcache04151915416476371="85 137 122"><a jquery18205432795217567932="47" href="http://www.biomedcentral.com/1471-2164/13/473/#ins7">7</a></sup>, <strong>Chulhong Kim</strong><sup sizset="false" sizcache04151915416476371="85 137 123"><a jquery18205432795217567932="48" href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup>, <strong>Tae-Hyung Kim</strong><sup sizset="false" sizcache04151915416476371="85 137 124"><a jquery18205432795217567932="49" href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup>, <strong>Seungwoo Hwang</strong><sup sizset="false" sizcache04151915416476371="85 137 125"><a jquery18205432795217567932="50" href="http://www.biomedcentral.com/1471-2164/13/473/#ins8">8</a></sup>, <strong>Jong Bhak</strong><sup sizset="false" sizcache04151915416476371="85 137 126"><a jquery18205432795217567932="51" href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup><sup sizset="false" sizcache04151915416476371="85 137 127"><a jquery18205432795217567932="52" href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup><sup>*</sup>, <strong>Hak-Kyo Lee</strong><sup sizset="false" sizcache04151915416476371="85 137 128"><a jquery18205432795217567932="53" href="http://www.biomedcentral.com/1471-2164/13/473/#ins1">1</a></sup><sup>*</sup> and <strong>Byung-Wook Cho</strong><sup sizset="false" sizcache04151915416476371="85 137 129"><a jquery18205432795217567932="54" href="http://www.biomedcentral.com/1471-2164/13/473/#ins5">5</a></sup><sup>*</sup></p>
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<div class="multipleins">
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<p><strong>Kyung-Do Park</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins1">1</a></sup><sup>&dagger;</sup>, <strong>Jongsun Park</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup><sup>&dagger;</sup>, <strong>Junsu Ko</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup><sup>&dagger;</sup>, <strong>Byung Chul Kim</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup>, <strong>Heui-Soo Kim</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins4">4</a></sup>, <strong>Kung Ahn</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins4">4</a></sup>, <strong>Kyoung-Tag Do</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins5">5</a></sup>, <strong>Hansol Choi</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup>, <strong>Hak-Min Kim</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup>, <strong>Sanghoon Song</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup>, <strong>Sunghoon Lee</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup>, <strong>Sungwoong Jho</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup>, <strong>Hong-Sik Kong</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins1">1</a></sup>, <strong>Young Mok Yang</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins6">6</a></sup>, <strong>Byung-Hak Jhun</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins7">7</a></sup>, <strong>Chulhong Kim</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup>, <strong>Tae-Hyung Kim</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup>, <strong>Seungwoo Hwang</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins8">8</a></sup>, <strong>Jong Bhak</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup><sup>*</sup>, <strong>Hak-Kyo Lee</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins1">1</a></sup><sup>*</sup> and <strong>Byung-Wook Cho</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins5">5</a></sup><sup>*</sup></p>
 
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</div>
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<p>&nbsp;</p>
 
<p>&nbsp;</p>

Revision as of 18:15, 3 June 2016

[ Transcriptomics class ] 

 

# Transcriptome? 

  = set of all mRNA molecules that are produced by the gemone, under specific circumstances in one cell or a population of cells 

  = can vary depending on environmental conditions

     ⇒ reflects genes which are being actively expressed with the exception of mRNA degradation 

Transcriptomics?

  = the study of transcriptome 

  = referred to as expression profiling

  = examines the expression level of mRNAs in a given cell population, often using high-throughput techniques based on DNA microarray technology. 

2. Relationship between genomics and transcriptomics

3. What are mRNAs?

4. How to measure RNA expression?

5. Relationship between Transcriptome and Proteome.

6. What is a UTR?

7. What is ncRNA ?

8. What is poly A ?

 

 

What to do:

1. Apply Biology learning procedure

 

 

Reference

http://www.biomedcentral.com/1471-2164/13/473

https://www.youtube.com/watch?v=UYJfM2-Y7y8  (Youtube by Illumina Gary Schroth)

 

 

 

Whole transcriptome analyses of six thoroughbred horses before and after exercise using RNA-Seq

Kyung-Do Park1, Jongsun Park2, Junsu Ko3, Byung Chul Kim23, Heui-Soo Kim4, Kung Ahn4, Kyoung-Tag Do5, Hansol Choi2, Hak-Min Kim2, Sanghoon Song3, Sunghoon Lee2, Sungwoong Jho2, Hong-Sik Kong1, Young Mok Yang6, Byung-Hak Jhun7, Chulhong Kim3, Tae-Hyung Kim3, Seungwoo Hwang8, Jong Bhak23*, Hak-Kyo Lee1* and Byung-Wook Cho5*