Difference between revisions of "ShinUnBeom"
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<p> Genomics is discipline of genetics for sequencing, assembling and analyzing the function and structure of entire genomes, not single genes.</p> | <p> Genomics is discipline of genetics for sequencing, assembling and analyzing the function and structure of entire genomes, not single genes.</p> | ||
<h3>Genome analysis</h3> | <h3>Genome analysis</h3> | ||
| − | < | + | <h5>DNA sequencing</h5> |
<p> First of all, select an organism of interest. Then sequence DNA fragments. There are several ways for sequencing DNA fragments; shotgun sequencing[http://en.wikipedia.org/wiki/Shotgun_sequencing], high-throughout sequencing, illumina sequencing[http://en.wikipedia.org/wiki/Illumina_dye_sequencing] and ion torrent[http://en.wikipedia.org/wiki/Ion_semiconductor_sequencing].</p> | <p> First of all, select an organism of interest. Then sequence DNA fragments. There are several ways for sequencing DNA fragments; shotgun sequencing[http://en.wikipedia.org/wiki/Shotgun_sequencing], high-throughout sequencing, illumina sequencing[http://en.wikipedia.org/wiki/Illumina_dye_sequencing] and ion torrent[http://en.wikipedia.org/wiki/Ion_semiconductor_sequencing].</p> | ||
| − | < | + | <h5>Sequence assembly</h5> |
<p> After sequencing DNA fragments, next step is assembling DNA fragments to rebuild original chromosome. There are two ways; de-novo assembly[http://en.wikipedia.org/wiki/De_novo_transcriptome_assembly] which assemble without any reference sequences, and comparative assembly which use sequences of similar organism for reference.</p> | <p> After sequencing DNA fragments, next step is assembling DNA fragments to rebuild original chromosome. There are two ways; de-novo assembly[http://en.wikipedia.org/wiki/De_novo_transcriptome_assembly] which assemble without any reference sequences, and comparative assembly which use sequences of similar organism for reference.</p> | ||
| − | < | + | <h5>Annotation</h5> |
| − | <p> Genome annotation is giving some biological information to sequences.</p> | + | <p> Genome annotation is giving some biological information to sequences. Main aspects for genome annotation is finding non-protein coding portion, identifying elements on genome and giving information to elements.</p> |
<h1>Transcriptomics </h1> | <h1>Transcriptomics </h1> | ||
<h1>Proteomics</h1> | <h1>Proteomics</h1> | ||
Revision as of 02:38, 15 May 2015
Contents
Principles of Bioinformatics
Bioprogramming
Genomics
Genomics is discipline of genetics for sequencing, assembling and analyzing the function and structure of entire genomes, not single genes.
Genome analysis
DNA sequencing
First of all, select an organism of interest. Then sequence DNA fragments. There are several ways for sequencing DNA fragments; shotgun sequencing[1], high-throughout sequencing, illumina sequencing[2] and ion torrent[3].
Sequence assembly
After sequencing DNA fragments, next step is assembling DNA fragments to rebuild original chromosome. There are two ways; de-novo assembly[4] which assemble without any reference sequences, and comparative assembly which use sequences of similar organism for reference.
Annotation
Genome annotation is giving some biological information to sequences. Main aspects for genome annotation is finding non-protein coding portion, identifying elements on genome and giving information to elements.
Transcriptomics
Proteomics
Epigenomics and Phenomics
Canceromics and Geromics
