Difference between pages "1" and "Trancriptomics Assay"
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imported>S (Created page with "<p><span style="font-size: small"> 1. <span class="reference-text"><span class="citation book">Winkler, HL (1920). <i>Verbreitung und Ursache der Parthenogenesis im Pflanzen...") |
imported>S (Created page with "<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-fami...") |
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| − | <p><span style="font-size: | + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">1.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">What is transcriptomics?<o:p></o:p></span></p> |
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">Thranscriptomics is discipline of biology that study about the set of all RNA moleculs, including mRNA, rRNA, tRNA and non-coding RNA transcribed in one cell or a population of cells<o:p></o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">2.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">Relationship between genomics and transcriptomics<o:p></o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">Transcriptomics is expressional genomics. <o:p></o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">3.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">What are mRNAs?<o:p></o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">mRNA is large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence if the protein products of gene expressing<o:p></o:p></span></p> | ||
| + | <p class="MsoListParagraph" style="margin: 0cm 0cm 8pt 40pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black; line-height: 107%"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">4.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">How to measure RNA expression?<o:p></o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif">RNA-seq method is frequently used for measuring RNA expression.<o:p></o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">5.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">Relationship between Transcriptome and Proteome.<o:p></o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt; line-height: 14.3pt; text-indent: 20.25pt; mso-char-indent-count: 1.5"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">Proteome is expressional Transcriptome. <o:p></o:p></span></p> | ||
| + | <p class="MsoListParagraph" style="margin: 0cm 0cm 8pt 40pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black; line-height: 107%"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">6.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">What is a UTR?<o:p></o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white; mso-bidi-font-weight: bold">Untranslated region </span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white">(or<span class="apple-converted-space"> </span><span style="mso-bidi-font-weight: bold">UTR</span>) refers to either of two sections, one on each side of a<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="Coding sequence" href="http://en.wikipedia.org/wiki/Coding_sequence"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; text-underline: none">coding sequence</span></a></span><span class="apple-converted-space"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white"> </span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white">on a strand of<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="MRNA" href="http://en.wikipedia.org/wiki/MRNA"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; text-underline: none">mRNA</span></a></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white">. If it is found on the 5' side, it is called the<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="Five prime untranslated region" href="http://en.wikipedia.org/wiki/Five_prime_untranslated_region"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; text-underline: none">5' UTR</span></a></span><span class="apple-converted-space"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white"> </span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white">(or<span class="apple-converted-space"> </span><span style="mso-bidi-font-weight: bold">leader sequence</span>), or if it is found on the 3' side, it is called the<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="Three prime untranslated region" href="http://en.wikipedia.org/wiki/Three_prime_untranslated_region"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; text-underline: none">3' UTR</span></a></span><span class="apple-converted-space"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white"> </span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white">(or<span class="apple-converted-space"> </span><span style="mso-bidi-font-weight: bold">trailer sequence</span>).<o:p></o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">7.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">What is ncRNA ?<o:p></o:p></span></p> | ||
| + | <p class="MsoNormal" style="margin: 0cm 0cm 8pt 15pt; mso-para-margin-left: 1.5gd"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white; line-height: 107%">A<span class="apple-converted-space"> </span><span style="mso-bidi-font-weight: bold">non-coding RNA</span><span class="apple-converted-space"> </span>(<span style="mso-bidi-font-weight: bold">ncRNA</span>) is a functional<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="RNA" href="http://en.wikipedia.org/wiki/RNA"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; line-height: 107%; text-underline: none">RNA</span></a></span><span class="apple-converted-space"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white; line-height: 107%"> </span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white; line-height: 107%">molecule that is not<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="Translation (genetics)" href="http://en.wikipedia.org/wiki/Translation_(genetics)"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; line-height: 107%; text-underline: none">translated</span></a></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white; line-height: 107%"> into a<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="Protein" href="http://en.wikipedia.org/wiki/Protein"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; line-height: 107%; text-underline: none">protein</span></a></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; line-height: 107%"><o:p></o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">8.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">What is poly A?<o:p></o:p></span></p> | ||
| + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">Poly A is long tail sequence of adenine nucleotides add it the 3’end of the pre-mRNA. This promotes export from the nucleus and translation, and protects the mRNA from degradation<o:p></o:p></span></p> | ||
| + | <p class="MsoNormal" style="margin: 0cm 0cm 8pt"><span lang="EN-US"><o:p><font size="2" face="맑은 고딕"> </font></o:p></span></p> | ||
| + | <p> </p> | ||
Revision as of 00:34, 21 May 2015
1. What is transcriptomics?<o:p></o:p>
Thranscriptomics is discipline of biology that study about the set of all RNA moleculs, including mRNA, rRNA, tRNA and non-coding RNA transcribed in one cell or a population of cells<o:p></o:p>
<o:p> </o:p>
2. Relationship between genomics and transcriptomics<o:p></o:p>
Transcriptomics is expressional genomics. <o:p></o:p>
<o:p> </o:p>
3. What are mRNAs?<o:p></o:p>
mRNA is large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence if the protein products of gene expressing<o:p></o:p>
<o:p> </o:p>
<o:p> </o:p>
4. How to measure RNA expression?<o:p></o:p>
RNA-seq method is frequently used for measuring RNA expression.<o:p></o:p>
<o:p> </o:p>
<o:p> </o:p>
5. Relationship between Transcriptome and Proteome.<o:p></o:p>
Proteome is expressional Transcriptome. <o:p></o:p>
<o:p> </o:p>
<o:p> </o:p>
6. What is a UTR?<o:p></o:p>
Untranslated region (or UTR) refers to either of two sections, one on each side of a coding sequence on a strand of mRNA. If it is found on the 5' side, it is called the 5' UTR (or leader sequence), or if it is found on the 3' side, it is called the 3' UTR (or trailer sequence).<o:p></o:p>
<o:p> </o:p>
7. What is ncRNA ?<o:p></o:p>
A non-coding RNA (ncRNA) is a functional RNA molecule that is not translated into a protein<o:p></o:p>
<o:p> </o:p>
<o:p> </o:p>
<o:p> </o:p>
<o:p> </o:p>
8. What is poly A?<o:p></o:p>
Poly A is long tail sequence of adenine nucleotides add it the 3’end of the pre-mRNA. This promotes export from the nucleus and translation, and protects the mRNA from degradation<o:p></o:p>
<o:p> </o:p>
