Difference between pages "The first Korean Genome Sequence" and "A Caucasian human genome was sequenced by Helicos single molecule sequencer"

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The first Korean genome sequence publicized (data) and published (research article) is from Gacheon Medical School and KRIBB (KOBIC) of Korea.<br />
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<font size="4">Single-molecule sequencing of an individual human genome</font><br />
 
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[[The first Korean genome sequence and analysis: Full genome sequencing for a socio-ethnic group]]<br />
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<font color="#0066cc">SRA009216<br />
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<p id="aug">Dmitry Pushkarev<sup><a title="affiliated with " href="http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.1561.html#a1"><font color="#0066cc">1</font></a>,</sup><sup><a title="affiliated with " href="http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.1561.html#a2"><font color="#0066cc">2</font></a></sup>, Norma F Neff<sup><a title="affiliated with " href="http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.1561.html#a1"><font color="#0066cc">1</font></a>,</sup><sup><a title="affiliated with " href="http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.1561.html#a2"><font color="#0066cc">2</font></a></sup> &amp; Stephen R Quake<sup><a title="affiliated with " href="http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.1561.html#a1"><font color="#0066cc">1</font></a></sup></p>
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<font color="#0066cc"><hr class="separator" />
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<p class="hidden"><font size="3">Billions of 24- to 70-bp reads (32 bp average)<br />
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<img style="BORDER-BOTTOM: 0px; BORDER-LEFT: 0px; VERTICAL-ALIGN: baseline; BORDER-TOP: 0px; BORDER-RIGHT: 0px" alt="approx" src="http://www.nature.com/__chars/math/special/sim/black/med/base/glyph.gif" />90% of the National Center for Biotechnology Information (NCBI) reference genome.<br />
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28<img style="BORDER-BOTTOM: 0px; BORDER-LEFT: 0px; VERTICAL-ALIGN: middle; BORDER-TOP: 0px; BORDER-RIGHT: 0px" alt="times" src="http://www.nature.com/__chars/math/special/times/black/med/base/glyph.gif" /> average coverage<br />
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by&nbsp;one sequencing instrument by a single operator with four data collection runs.&nbsp;<br />
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Single-molecule sequencing enabled analysis of human genomic information without the need for cloning, amplification or ligation.&nbsp;<br />
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determined <img style="BORDER-BOTTOM: 0px; BORDER-LEFT: 0px; VERTICAL-ALIGN: baseline; BORDER-TOP: 0px; BORDER-RIGHT: 0px" alt="approx" src="http://www.nature.com/__chars/math/special/sim/black/med/base/glyph.gif" />2.8 million single nucleotide polymorphisms (SNPs) with a false-positive rate of less than 1% as validated by Sanger sequencing and 99.8% concordance with SNP genotyping arrays.&nbsp;<br />
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identified 752 regions of copy number variation by analyzing coverage depth alone and validated 27 of these using digital PCR.&nbsp;<br />
 
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NCBI accession number: [[SRA008175]]<br />
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Nature Biotechnology</font><br />
 
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<a href="http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.1561.html">http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.1561.html</a>.<br />
 
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<font size="4">See also<br />
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[[Helicos Biosciences]]<br />
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</font>The second Korean genome accession number:&nbsp; [[SRA008370]]
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Revision as of 05:16, 13 August 2009

Single-molecule sequencing of an individual human genome

SRA009216

Dmitry Pushkarev1,2, Norma F Neff1,2 & Stephen R Quake1

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