View source for About the resaon why the helicase is not used in PCR

<p>Helicase is the enzyme that can denature the double strand structure of DNA into two single strands. It is crucially needed during DNA replication due to its denaturing function. Then the question is arisen. Why isn&#39;t it used for process of PCR even though they need to denature the double strand as well? Instead of using helicase, since PCR has heating phase&nbsp;to break the hydrogen bonds between double strand DNA but&nbsp;it additionally requires special Taq polymerase that can&nbsp;endure the heat. There would be clear reasons why researchers go long way around like this. So I supposed a reason for avoiding the helicase : there would be more complicating mechanism needed for using helicase.</p>

<p>Actually after the helicase denature the dsDNA into ssDNA, single strand binding protein(SSB protein) should bind&nbsp;&nbsp;to ssDNA to protect ssDNA and prevent the recombining.</p>