Changes
no edit summary
<p style="margin-left: 40px"> RNA is classified into two categories, coding RNA and non coding RNA. Messenger RNA is included in the coding RNA. It means that they encode amino acid sequences, so they act as template for protein synthesis. It consists of 5’ cap, 5’ UTR, coding sequence, 3’ UTR, Poly-A tail.</p>
<p> <strong>How to measure mRNA expression?</strong></p> <p style="margin-left: 40px">I learned from the lecture, and among them I searched about RNA-seq and microarray. RNA-seq is deep-sequencing technologies. First ome portion or total RNA is extracted from the cell, and they are converted to the cDNA. They are sequenced in a high-throughput manner by using Illumina IG, Applied Biosystem SOLiD, and so on, so this is expensive. Microarray is used to measure expression level of some genes, and for genotyping. At the solid surface, probe which is short sequence of RNA is anchored, and the RNA which have complementary to the probe is hybridized with the probe. Then, it is quantified by using fluoroscent. So, microarray only measure the expression of gene which is complementary to the probe. It can't detect novel transcripts, gene fusion, but RNA-seq can do this. Also, RNA-seq is more sensitive and specific.</p>
<p><strong> Relationship between transcriptome and proteome.</strong></p>