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<p>After this class, I had a question. Is the cDNA is important in transcriptomics? Why cDNA is used?</p>
<p>The answer is yes. For example, to using microarray, complementary sequences for mRNA is needed to attach and analyze the target mRNA. cDNA is in complementary relations with mRNA, hence, cDNA is important. However, not using cDNA technique is also exist, such as single molecule Direct RNA Sequencing.</p> <p>Then, Why don't use just DNA sequence instead of cDNA?</p> <p>I guess, it's because DNA is consist of both introns and exons, but mRNA is just consist of exons, usually.</p>
<p>In the Jong’s PPT, there was slide about life. I was very impressed about that slide when I saw it first, so I thought about life. In my definition, life is linkage. People are linked with other person or not, like enzymes binds with substrates or not.</p>