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<p><strong>Introduction</strong></p>
<p>The genetic code of all organisms encodes just 20 common amino acids. However, these 20 amino acids, canonical amino acids, can be modified by post-translation modification(PTM) process. Through the PTM tons of various amino acids are formed and they have their own important chemical biological functions in organism. Among those PTM, some of PTM’s function was still not discovered well. One example of that is N-phosphorylation. N-phosphorylation means phosphorylation to N group in side chain of His, Lys or Arg. Those N-phosphorylation was still quite not explored because of its low stability in N-phosphate bond.</p>
<p>Among those PTMDNA sequences are transcripted to mRNA and then translated to amino acids. During the translation, each three mRNA sequence is assigned to specific amino acid. In 2001 science, some one interesting paper was published; Expanding genetic code of PTMescherichia coli. In this paper, they generated&rsquonbsp;s function was still not discovered well. One example of that is Nunique tRNA/aminoacyl-phosphorylation. NtRNA synthetase pair to express non-phosphorylation means phosphorylation canical amino acid at the site corresponed to N group amber codon in side chain of HismRNA. In this way, Lys or Arg. Those Nthey conducted in vivo incorporation O-methyl-phosphorylation was still quite not explored because of its low stability tyrosine into protein in NE-phosphate bondcoli.</p>
<p>DNA sequences are transcripted to mRNA and then translated Then why don't we improve this technique to express some unstable non-canonical amino acids. During the translation, each three mRNA sequence is assigned to specific amino acid. In 2001, one interesting?</p>
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<p><strong>Body</strong></p>
<p>Let's focus on N-phosphorylated amino acids as a subject for expanding genetic code. There were some previous researches about pHis and it is known that pHis conduct stress regulation system, so called two-component system, in E-coli. But still we don't know much about function of pHis in eukaryotic cell. Compared to other phosphorylated amino acids such as pTyr, pSer and pThr, N-phosphorylated amino acid were less understood because of acid-labile property.</p>
<p><img alt="" src="/ckfinder/userfiles/images/%EA%B7%B8%EB%A6%BC1(1).png" style="height:111px; width:200px" /></p>
<p>N-phosphorylated amino acid are fragile because it undergoes hydrolysis in acidic or even neutral pH. Therefore most of in vitro phosphorylation to get pHis, pAsp and pArg in concucted at basic condition using potassium phosphoramidate.</p>
<p>Amber codon suppression is a technique to incorporate non-canonical amino aicds in protein by developing novel tRNA and aminoacyl-tRNA synthetase pair. Then if we can develop tRNA which carries N-phosphorylated amino acid in basic condition and develop matched aminoacyl tRNA synthetase, manual in vivo incorporation of N-phosphorylated amino acid in protein would be possible.</p>
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