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Self evaluation paper

18 bytes added, 00:10, 5 December 2016
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<p style="margin-left: 38pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Correspondence: <a href="mailto:woobin512@unist.ac.kr">woobin512@unist.ac.kr</a></p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
<p style="margin-left: 38pt">&nbsp;</p>
<p>Once we treat mETP-SpyTag protein in to differentiated adipocyte, mETP will connect with unknown target protein. Because of covalent interaction between SpyCatcher-APEX2 <strong>(Reddington SC. et al. 2015), </strong>SpyTag will make covalent bond with SpyCatcher and finally mETP-SpyTag-SpyCarcher-APEX2 appear. Then, by treating DBP(Desthiobiotin-Phenol) and H2O2, DBP will become radical and biotinalize interactors.<strong> (Lam SS. et al. 2015) </strong>By using the technique of Mass-Spectrometry with streptavidin beads,<strong> (Ruedi. et al. 2016) </strong>we can enrichment unknown protein.</p>
<p>&nbsp;[[File:Self3.png|400px]]</p>
<p><strong>Figure 1.b.</strong> <strong>The targeted and untargeted workflow for LC/MS-based metabolomics </strong></p>
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