<ul> <li>Sanger sequencing by capillary electrophoresis is the gold-standard DNA sequencing technique.</li> <li>Based on the selective incorporation of chain-terminating ddNTP by DNA polymerase during in vitro DNA replication.</li> <li>requires a single-stranded DNA template, a DNA&nbsp;primer, a&nbsp;DNA polymerase, normal deoxynucleosidetriphosphates (dNTPs), and modified dispan style="font-deoxynucleosidetriphosphates (ddNTPs)</li> <li>resulted DNA fragments are heat denatured and separated by size using gel electrophoresis</li> <li>DNA polymerases copy single-stranded DNA templates by adding nucleotides to a growing chain. Chain elongation occurs at the 3&#39:22px; end of a primer, an oligonucleotide that anneals to the template. The deoxynucleotides added to the extension product are selected by base-pair matching to the template.</li"> '''1<lisup>Thermal cycling -&gt; purification -&gt; capillary electrophoresisst</lisup>sequencing – Sanger sequencing'''</ulspan>

<p style="margin*'''Sanger sequencing by capillary electrophoresis is the gold-left:38standard DNA sequencing technique.0pt"><''' *'''Based on the selective incorporation of chain-terminating ddNTP by DNA polymerase during in vitro DNA replication.''' *'''requires a single-stranded DNA template, a DNA&nbsp;primer, a href="http://media.invitrogen.com.edgesuite.net/ab/applications&nbsp;DNA polymerase, normal deoxynucleosidetriphosphates (dNTPs), and modified di-technologies/pharmadeoxynucleosidetriphosphates (ddNTPs)''' *'''resulted DNA fragments are heat denatured and separated by size using gel electrophoresis''' *'''DNA polymerases copy single-biotherapeutics/DNA_sequencingstranded DNA templates by adding nucleotides to a growing chain.swf">http://mediaChain elongation occurs at the 3' end of a primer, an oligonucleotide that anneals to the template.invitrogen.com.edgesuiteThe deoxynucleotides added to the extension product are selected by base-pair matching to the template.net/ab/applications''' *'''Thermal cycling -technologies/pharma> purification -biotherapeutics/DNA_sequencing.swf</a></p>capillary electrophoresis'''

<p style="margin[http://media.invitrogen.com.edgesuite.net/ab/applications-technologies/pharma-leftbiotherapeutics/DNA_sequencing.swf '''http:38//media.invitrogen.com.0pt"><a href="edgesuite.net/ab/applications-te'''chnologies/pharma-biotherapeutics/DNA_sequencing.swf] [https://en.wikipedia.org/wiki/Sanger_sequencing">https://en.wikipedia.org/wiki/Sanger_sequencing</a></p>]