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Created page with "<p>-NCBI Blast tutorial : https://www.youtube.com/watch?v=HXEpBnUbAMo</p> <p>- Bioinformatics practical 4 multiple sequence alignment using ClustalW : https://www..."
<p>-NCBI Blast tutorial : https://www.youtube.com/watch?v=HXEpBnUbAMo</p>
<p>- Bioinformatics practical 4 multiple sequence alignment using ClustalW : https://www.youtube.com/watch?v=tq-bWYVnQCM</p>
<p>1. Find the protein in NCBI and retrieve the sequence and save it in computer</p>
<p>2. Run BLAST to find similar sequences and retrieve some similar sequences in FASTA format</p>
<p>3. Look for the presence of conseved domain</p>
<p>4. Go for the multiple sequence alignment with 10 of the retrieved sequence</p>
<p>5. Construct the phylogenetic tree with the sequence alignment.</p>
<p>6. Predict the secondary structure of the protein</p>
<p>7. Retrieved the 3D structure of protein from PDB</p>
<p>8. View the 3D structure of protein in Jmol</p>
<p><img alt="amino acid table에 대한 이미지 검색결과" src="https://upload.wikimedia.org/wikipedia/commons/thumb/5/51/Molecular_structures_of_the_21_proteinogenic_amino_acids.svg/770px-Molecular_structures_of_the_21_proteinogenic_amino_acids.svg.png" /></p>
<p>amino acid table</p>
<p>- clustal W : https://www.ebi.ac.uk/Tools/msa/clustalo/</p>
<p>- Bioinformatics practical 4 multiple sequence alignment using ClustalW : https://www.youtube.com/watch?v=tq-bWYVnQCM</p>
<p>1. Find the protein in NCBI and retrieve the sequence and save it in computer</p>
<p>2. Run BLAST to find similar sequences and retrieve some similar sequences in FASTA format</p>
<p>3. Look for the presence of conseved domain</p>
<p>4. Go for the multiple sequence alignment with 10 of the retrieved sequence</p>
<p>5. Construct the phylogenetic tree with the sequence alignment.</p>
<p>6. Predict the secondary structure of the protein</p>
<p>7. Retrieved the 3D structure of protein from PDB</p>
<p>8. View the 3D structure of protein in Jmol</p>
<p><img alt="amino acid table에 대한 이미지 검색결과" src="https://upload.wikimedia.org/wikipedia/commons/thumb/5/51/Molecular_structures_of_the_21_proteinogenic_amino_acids.svg/770px-Molecular_structures_of_the_21_proteinogenic_amino_acids.svg.png" /></p>
<p>amino acid table</p>
<p>- clustal W : https://www.ebi.ac.uk/Tools/msa/clustalo/</p>
