<h1><span style="font -size="4:12px">Single-molecule sequencing of an individual human genome1. ATAD5&nbsp;ATPase family, AAA domain containing 5 [&nbsp;</fontem>Homo sapiens<br /em>&nbsp;(human) ]<br /span><font color="#0066cc">SRA009216<br /h1></font><br /><p id="aug">Dmitry Pushkarev<sup><a titlespan style="affiliated with " href="httpfont-size://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.1561.html#a112px"><font color="#0066cc">1Gene ID: 79915, updated on 26-May-2016</fontspan></ap>, </supp><sup><a titlespan style="affiliated with " href="httpfont-size://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.1561.html#a212px"><font color="#0066cc">2&lt;i want to understand about PCNA and ATAD5 role. so pick that&gt;</fontspan></ap> </supp>, Norma F Neff<sup><a titlespan style="affiliated with " href=font-size:12px">source comes from. http://www.naturencbi.nlm.nih.comgov/nbtprotein/journal26080431?report=fasta</vaop/ncurrent/abs/nbt.1561.html#a1"><font color="#0066cc"span>1</fontp> </ah1>,ATPase family AAA domain-containing protein 5 [Homo sapiens]</suph1> <supp><a title="affiliated with " href="httpNCBI Reference Sequence://wwwNP_079133.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.1561.html#a2"><font color="#0066cc">23</fontp> </a></suppre> &ampgt; Stephen R Quake<sup><a title="affiliated with " href="http://wwwgi|26080431|ref|NP_079133.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.1561.html#a1"><font color="#0066cc">1</font>3| ATPase family AAA domain-containing protein 5 [Homo sapiens]MVGVLAMAAAAAPPPVKDCEIEPCKKRKKDDDTSTCKTITKYLSPLGKTRDRVFAPPKPSNILDYFRKTSPTNEKTQLGKECKIKSPESVPVDSNKDCTTPLEMFSNVEFKKKRKRVNLSHQLNNIKTENEAPIEISSDDSKEDYSLNNDFVESSTSVLRYKKQVEVLAENIQDTKSQPNTMTSLQNSKKVNPKQGTTKNDFKKLRKRKCRDVVDLSESLPLAEELNLLKKDGKDTKQMENTTSHANSRDNVTEAAQLNDSIITVSYEEFLKSHKENKVEEIPDSTMSICVPSETVDEIVKSGYISESENSEISQQVRFKTVTVLAQVHPIPPKKTGKIPRIFLKQKQFEMENSLSDPENEQTVQKRKSNVVIQEEELELAVLEAGSSEAVKPKCTLEERQQFMKAFRQPASDALKNGVKKSSDKQKDLNEKCLYEVGRDDNSKKIMENSGIQMVSKNGNLQLHTDKGSFLKEKNKKLKKKNKKTLDTGAIPGKNREGNTQKKETTFFLKEKQYQNRMSLRQRKTEFFKSSTLFNNESLVYEDIANDDLLKVSSLCNNNKLSRKTSIPVKDIKLTQSKAESEASLLNVSTPKSTRRSGRISSTPTTETIRGIDSDDVQDNSQLKASTQKAANLSEKHSLYTAELITVPFDSESPIRMKFTRISTPKKSKKKSNKRSEKSEATDGGFTSQIRKASNTSKNISKAKQLIEKAKALHISRSKVTEEIAIPLRRSSRHQTLPERKKLSETEDSVIIIDSSPTALKHPEKNQKKLQCLNDVLGKKLNTSTKNVPGKMKVAPLFLVRKAQKAADPVPSFDESSQDTSEKSQDCDVQCKAKRDFLMSGLPDLLKRQIAKKAAALDVYNAVSTSFQRVVHVQQKDDGCCLWHLKPPSCPLLTKFKELNTKVIDLSKCGIALGEFSTLNSKLKSGNSAAVFMRTRKEFTEEVRNLLLEEIRWSNPEFSLKKYFPLLLKKQIEHQVLSSECHSKQELEADVSHKETKRKLVEAENSKSKRKKPNEYSKNLEKTNRKSEELSKRNNSSGIKLDSSKDSGTEDMLWTEKYQPQTASELIGNELAIKKLHSWLKDWKRRAELEERQNLKGKRDEKHEDFSGGIDFKGSSDDEEESRLCNTVLITGPTGVGKTAAVYACAQELGFKIFEVNASSQRSGRQILSQLKEATQSHQVDKQGVNSQKPCFFNSYYIGKSPKKISSPKKVVTSPRKVPPPSPKSSGPKRALPPKTLANYFKVSPKPKNNEEIGMLLENNKGIKNSFEQKQITQTKSTNATNSNVKDVGAEEPSRKNATSLILFEEVDVIFDEDAGFLNAIKTFMATTKRPVILTTSDPTFSLMFDGCFEEIKFSTPSLLNVASYLQMICLTENFRTDVKDFVTLLTANTCDIRKSILYLQFWIRSGGGVLEERPLTLYRGNSRNVQLVCSEHGLDNKIYPKNTKKKRVDLPKCDSGCAETLFGLKNIFSPSEDLFSFLKHKITMKEEWHKFIQLLTEFQMRNVDFLYSNLEFILPLPVDTIPETKNFCGPSVTVDASAATKSMNCLARKHSEREQPLKKSQKKKQKKTLVILDDSDLFDTDLDFPDQSISLSSVSSSSNAEESKTGDEESKARDKGNNPETKKSIPCPPKTTAGKKCSALVSHCLNSLSEFMDNMSFLDALLTDVREQNKYGRNDFSWTNGKVTSGLCDEFSLESNDGWTSQSSGELKAAAEALSFTKCSSAISKALETLNSCKKLGRDPTNDLTFYVSQKRNNVYFSQSAANLDNAWKRISVIKSVFSSRSLLYVGNRQASIIEYLPTLRNICKTEKLKEQGKSKRRFLHYFEGIHLDIPKETVNTLAADFP</apre> </supp>&nbsp;</p> <p class="caff"><font color="#0066cc"></font>&nbsp;</p><font color="#0066cc"><hr class="separator" /></font><p class="hidden"><font size="3">Billions of 24- to 70-bp reads (32 bp average)<br /><img style="BORDER-BOTTOMresult : 0px; BORDER-LEFT: 0px; VERTICAL-ALIGN: baseline; BORDER-TOP: 0px&nbsp; BORDER-RIGHT: 0px" alt="approx" src="http://wwwzhanglab.natureccmb.com/__chars/math/special/sim/black/med/base/glyph.gif" />90% of the National Center for Biotechnology Information (NCBI) reference genomeumich.<br edu/>28<img style="BORDER-BOTTOM: 0px; BORDER-LEFT: 0px; VERTICALI-ALIGN: middle; BORDER-TOP: 0px; BORDER-RIGHT: 0px" alt="times" src="http://www.nature.comTASSER/__charsoutput/mathS274747/special/times/black/med/base/glyph.gif" </p> average coverage <br /p>by&nbsp;one sequencing instrument by a single operator with four data collection runs.&nbsp;<br />Single-molecule sequencing enabled analysis of human genomic information without the need for cloning, amplification or ligation.&nbsp;<br />determined <img style="BORDER-BOTTOM: 0px&nbsp; BORDER-LEFT: 0px&nbsp; VERTICAL-ALIGN: baseline&nbsp; BORDER-TOP: 0px; BORDER-RIGHT: 0px" alt="approx" src="http://wwwzhanglab.natureccmb.com/__chars/math/special/sim/black/med/base/glyph.gif" umich.edu/>2.8 million single nucleotide polymorphisms (SNPs) with a falseI-positive rate of less than 1% as validated by Sanger sequencing and 99.8% concordance with SNP genotyping arrays.&nbsp;<br TASSER/>identified 752 regions of copy number variation by analyzing coverage depth alone and validated 27 of these using digital PCR.&nbsp;<br output/><br S274747/><br /p>Nature Biotechnology</font><br /><br /p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<a href="http://wwwzhanglab.ccmb.med.natureumich.comedu/nbt/journalI-TASSER/vaopoutput/ncurrentS274747/abs/nbtS274747_results.1561tar.htmlbz2">http://wwwzhanglab.ccmb.med.natureumich.comedu/nbt/journalI-TASSER/vaopoutput/ncurrentS274747/abs/nbtS274747_results.1561tar.htmlbz2</a>.<br /><br />[[Helicos Biosciences]]<br /></p>