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<p><strong>Expanding genetic code for unstable non-canonical amino acids by using stop (amber) codon suppression technique</strong></p>
<p>20141541 Jihwan Jeon</p>
<p>DNA sequences are transcripted to mRNA and then translated to amino acids. During the translation, each three mRNA sequence is assigned to specific amino acid. In 2001 science, one interesting paper was published; Expanding genetic code of escherichia coli. In this paper, they generated unique tRNA/aminoacyl-tRNA synthetase pair to express non-canical amino acid at the site corresponed to amber codon in mRNA. In this way, they conducted in vivo incorporation O-methyl-tyrosine into protein in E-coli.</p>
<p><img alt="" src="/ckfinder/userfiles/images/%EA%B7%B8%EB%A6%BC2(1).jpg" style="height:51px154px; width:200px600px" /></p>
<p>Then why don't we improve this technique to express some unstable non-canonical amino acids?</p>
<p><strong>Colclusion</strong></p>
<p>Chemical probe to catch N-phosphorylated protein have been developing and it is attractive way to figure out biological function of N-phosphorylation. I conducted reaearch about finding chemical probe of pLys and pAsp(althought it is not N-phosphorylation, it is also not stable) using antibody. This chemical approach to unstable phosphorylation is quite attractive but not easy. So I proposed another way to investigate the function of unstable non-canonical amino acids by developing tRNA and aminoacyl tRNA synthetase. If we can manage N-phosphorylated protein in organism, then it would be much easier, even after developing chemical probe for those amino acids, to conduct biological assay.</p>
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<p><strong>References</strong></p>
<p>Kim Wals and HuibOvaa, Front. Chem., 01 April 2014, Unnatural amino acid incorporation in E. coli</p> <p>P. G. Besant, P. V. Attwood, M. J. Piggott. Current Protein & Peptide Science, Volume 10 , Issue 6 , 2009, Focus on Phosphoarginine and Phospholysine</p> <p>Lei Wang, Ansgar Brock, Brad Herberich, Peter G. Schultz, Science, Volume 292, 20 April 2001, Expanding the Genetic Code of Escherichia coli</p>
