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<p style="margin-left: 40px;">This Review focuses on commercially available technologies from<strong> Roche/454, Illumina/Solexa, Life/APG and Helicos BioSciences, the Polonator instrument </strong>and the near-term technology of<strong> Pacific Biosciences, </strong></p>

<p style="margin-left: 40px;">Here, I present a technical review of <strong> template preparation, sequencing and imaging, genome alignment and assembly</strong>, and current NGS platform performance to provide guidance on how<br /em>how these technologies work and how they may be applied<br />to important biological questions. ​</em></p>

<p style="margin-left: 40px"><img alt="" src="/ckfinder/userfiles/images/Picture2(1).png" style="height:13px;width:200px"/>&nbsp;</p>

<p style="margin-left: 40px80px;">&nbsp;Sequencing technologies include a number of methods<br />that are grouped broadly as <strong>template preparation,<br />sequencing and imaging, and data analysis.</strong></p>

<ul> <li style="margin-left: 80px;"> <p><em>There are two methods used in preparing templates for NGS reactions</em>: <strong>clonally amplified templates</strong> originating from single DNA molecules, and<strong> single DNA molecule templates</strong></p > </li> <li style="margin-left: 40px80px;">&nbsp; <p><em>Sequencing methods are classified as</em> <strong>cyclic reversible termination (CRT), single-nucleotide addition (SNA) and real-time sequencing.</strong> ​</p> </li></ul>

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<p style="margin-left: 40px">&nbsp;</p> <p style="margin-left:40px">&nbsp;</p> <p style="margin-left:40px">&nbsp;</p>